reference strains mycobacterium mageritense dsm 44476 t Search Results


immunocap miscellaneous allergens, cotton  (Thermo Fisher)
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Quantify specific IgE antibodies with ImmunoCAP™ Miscellaneous Allergens, essential tools to aid in the diagnosis of allergic conditions. IgE antibodies appear in human serum and plasma as a result of sensitization to a specific allergen.
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mycobacterium mageritense dsm 44476  (ATCC)
93
ATCC mycobacterium mageritense dsm 44476
Mycobacterium Mageritense Dsm 44476, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium mageritense dsm 44476t  (DSMZ)
93
DSMZ mycobacterium mageritense dsm 44476t
Mycobacterium Mageritense Dsm 44476t, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ab19136 rrid ab 444764 tbk1 cst cat 38 066  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc ab19136 rrid ab 444764 tbk1 cst cat 38 066
Figure 1. Increased activation and mitochondrial localization of <t>TBK1</t> in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.
Ab19136 Rrid Ab 444764 Tbk1 Cst Cat 38 066, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m heidelbergense dsm 44471t  (ATCC)
90
ATCC m heidelbergense dsm 44471t
Figure 1. Increased activation and mitochondrial localization of <t>TBK1</t> in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.
M Heidelbergense Dsm 44471t, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+strains+mycobacterium+mageritense+dsm+44476+t/Rhizopus+oryzae+Went+et+Prinsen+Geerligs/pm20693362-10-31-44
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nbp2-44476  (Bio-Techne corporation)
89
Bio-Techne corporation nbp2-44476
Figure 1. Increased activation and mitochondrial localization of <t>TBK1</t> in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.
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ugt1a sirna  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ugt1a sirna
Figure 1. Increased activation and mitochondrial localization of <t>TBK1</t> in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.
Ugt1a Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+strains+mycobacterium+mageritense+dsm+44476+t/UGT1A+siRNA/pm29444544-35-7-9
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(s)-methyl 3-amino-3-(3-nitrophenyl)propanoate hydrochloride  (Biosynth Carbosynth)
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2,3-dihydro-2-[3-(2-phenoxyethoxy)phenyl]-4(1h)-quinazolinone-d5  (Toronto Research Chemicals)
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bv711 hamster anti mouse vδ 6 3 2 tcr  (BD Biosciences)
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6-chloropurine-9-beta-d-(3’ 5’-di-o-benzoyl-2’-deoxy-2’-fluoro)-arabinoriboside  (Biosynth Carbosynth)
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l-menthol  (Biosynth Carbosynth)
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Image Search Results


Figure 1. Increased activation and mitochondrial localization of TBK1 in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence-Associated Pathologies.

doi: 10.1002/advs.202404109

Figure Lengend Snippet: Figure 1. Increased activation and mitochondrial localization of TBK1 in senescent cells. A) SA-𝛽-gal staining of normal and senescent cells in various senescent cell models (n = 3). P: passages, Dox: Doxorubicin (150 nm), WT: wild type, HGPS: Hutchinson–Gilford progeria syndrome. Scale bars, 50 μm. B) Immunoblot analysis of phosphorylated TBK1 (pS172-TBK1) and TBK1 in normal or senescent cells (n = 3). C) Confocal imaging of TBK1 (red) and Tom20 (green) co-localization in normal or senescent cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. D) Transmission electron microscopy (TEM) images of TBK1 labeled by antibody-conjugated gold particles in normal or senescent cells (n = 3). m: mitochondria. Scale bars, 100 nm. E) Immunoblot analysis of p-TBK1 and TBK1 in the mitochondrial fraction of normal or senescent cells (n = 3). Tom40: mitochondria marker, GAPDH: whole cell marker.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATAD3A Abnova Cat#H00055210; RRID:AB_10718149 p-Ser/Thr Abcam Cat# ab17464; RRID:AB_443891 p21 Abcam Cat# ab109199; RRID:AB_10861551 p16 Abcam Cat# ab108349; RRID:AB_10858268 HA Abcam Cat# ab9110; RRID:AB_307019 LAMP1 Abcam Cat# ab25630; RRID:AB_470708 Ki-67 Abcam Cat# ab15580; RRID:AB_443209 VeriBlot for IP Detection Reagent (HRP) Abcam Cat# ab131366; RRID:AB_2892718 p-Parkin Abcam Cat# ab ab315376 HSC-70 Abcam Cat# ab19136 , RRID:AB_444764 TBK1 CST Cat# 38 066; RRID:AB_2827657 PLK1 CST Cat# 4513; RRID:AB_2167409 PKCδ CST Cat# 9616; RRID:AB_10949973 Cleaved caspase3 CST Cat# 9664; RRID:AB_2070042 PKM2 CST Cat# 4053; RRID:AB_1904096 PDH CST Cat# 3205; RRID:AB_2162926 GAPDH CST Cat# 2118; RRID:AB_561053 Tom20 Santa Cruz Cat# sc-11415; RRID:AB_2207533 Tim23 Santa Cruz Cat# sc-514463; RRID:AB_2923126 Tom40 Santa Cruz Cat# sc-365467; RRID:AB_10847086 GFP Santa Cruz Cat# sc-9996; RRID:AB_627695 TBK1 Santa Cruz Cat# sc-398366 β-Actin Santa Cruz Cat# sc-8432; RRID:AB_626630 Ubiquitin Santa Cruz Cat# sc-8017; RRID:AB_628423 OPTN Santa Cruz Cat# sc-166576; RRID:AB_2156554 p53 Santa Cruz Cat# sc-126; RRID:AB_628082 Flag Sigma Cat# F3165; RRID:AB_259529 Mitofilin Novus Cat#NB100-74461 ANT1 Proteintech Cat# 30631-1-AP Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31460 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11008; RRID:AB_143165 Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11001; RRID:AB_2534069 Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-21207; RRID:AB_141637 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-11037; RRID:AB_2534095 Chemicals Doxorubicin Selleck Cat# E2516 Recombinant TBK1 Protein Abcam Cat# ab85276 Protease Inhibitor Cocktail Roche Cat# P8340 Phosphatase inhibitor cocktail Roche Cat# PHOSS-RO (Continued) Adv.

Techniques: Activation Assay, Staining, Western Blot, Imaging, Transmission Assay, Electron Microscopy, Labeling, Marker

Figure 2. TBK1 phosphorylates ATAD3A at Ser321 to promote cellular senescence. A) Immunoblot analysis of the interaction between endogenous ATAD3A and TBK1 in normal or senescent A549 and WI-38 cells (n = 3). B) Confocal imaging of ATAD3A (green) and TBK1 (red) co-localization in normal or senescent A549 and WI-38 cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. C) Immunoblot analysis of phosphorylated ATAD3A in senescent cells using a p-Ser- and p-Thr-specific antibody (n = 3). D) In vitro TBK1 kinase assay using recombinant TBK1 and Flag-ATAD3A purified from 293T cells as the substrate (n = 3). E) Listing of 2 ATAD3A Ser phosphorylation sites (highlighted in red) identified

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence-Associated Pathologies.

doi: 10.1002/advs.202404109

Figure Lengend Snippet: Figure 2. TBK1 phosphorylates ATAD3A at Ser321 to promote cellular senescence. A) Immunoblot analysis of the interaction between endogenous ATAD3A and TBK1 in normal or senescent A549 and WI-38 cells (n = 3). B) Confocal imaging of ATAD3A (green) and TBK1 (red) co-localization in normal or senescent A549 and WI-38 cells (n = 3). Areas outlined by squares in the merged images are enlarged at right. Scale bars, 5 μm. C) Immunoblot analysis of phosphorylated ATAD3A in senescent cells using a p-Ser- and p-Thr-specific antibody (n = 3). D) In vitro TBK1 kinase assay using recombinant TBK1 and Flag-ATAD3A purified from 293T cells as the substrate (n = 3). E) Listing of 2 ATAD3A Ser phosphorylation sites (highlighted in red) identified

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATAD3A Abnova Cat#H00055210; RRID:AB_10718149 p-Ser/Thr Abcam Cat# ab17464; RRID:AB_443891 p21 Abcam Cat# ab109199; RRID:AB_10861551 p16 Abcam Cat# ab108349; RRID:AB_10858268 HA Abcam Cat# ab9110; RRID:AB_307019 LAMP1 Abcam Cat# ab25630; RRID:AB_470708 Ki-67 Abcam Cat# ab15580; RRID:AB_443209 VeriBlot for IP Detection Reagent (HRP) Abcam Cat# ab131366; RRID:AB_2892718 p-Parkin Abcam Cat# ab ab315376 HSC-70 Abcam Cat# ab19136 , RRID:AB_444764 TBK1 CST Cat# 38 066; RRID:AB_2827657 PLK1 CST Cat# 4513; RRID:AB_2167409 PKCδ CST Cat# 9616; RRID:AB_10949973 Cleaved caspase3 CST Cat# 9664; RRID:AB_2070042 PKM2 CST Cat# 4053; RRID:AB_1904096 PDH CST Cat# 3205; RRID:AB_2162926 GAPDH CST Cat# 2118; RRID:AB_561053 Tom20 Santa Cruz Cat# sc-11415; RRID:AB_2207533 Tim23 Santa Cruz Cat# sc-514463; RRID:AB_2923126 Tom40 Santa Cruz Cat# sc-365467; RRID:AB_10847086 GFP Santa Cruz Cat# sc-9996; RRID:AB_627695 TBK1 Santa Cruz Cat# sc-398366 β-Actin Santa Cruz Cat# sc-8432; RRID:AB_626630 Ubiquitin Santa Cruz Cat# sc-8017; RRID:AB_628423 OPTN Santa Cruz Cat# sc-166576; RRID:AB_2156554 p53 Santa Cruz Cat# sc-126; RRID:AB_628082 Flag Sigma Cat# F3165; RRID:AB_259529 Mitofilin Novus Cat#NB100-74461 ANT1 Proteintech Cat# 30631-1-AP Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31460 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11008; RRID:AB_143165 Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11001; RRID:AB_2534069 Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-21207; RRID:AB_141637 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-11037; RRID:AB_2534095 Chemicals Doxorubicin Selleck Cat# E2516 Recombinant TBK1 Protein Abcam Cat# ab85276 Protease Inhibitor Cocktail Roche Cat# P8340 Phosphatase inhibitor cocktail Roche Cat# PHOSS-RO (Continued) Adv.

Techniques: Western Blot, Imaging, In Vitro, Kinase Assay, Recombinant, Phospho-proteomics

Figure 7. TBK1-ATAD3A-Pink1 axis inhibits mitophagy and promotes cellular senescence. A) SA-𝛽-gal staining of indicated A549 cells (n = 3). Cells were initially induced with DMSO or doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. B) Immunoblot analysis of p53, p21, and Pink1 in the cells described in A (n = 3). C) Confocal imaging of Tom20 (green) and LAMP1 (red) in indicated cells (n = 3). Cells were initially induced with doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. Areas outlined by squares in the merged images are enlarged at right. Scale bars, 2 μm. D) SA-𝛽-gal staining of NC, shTBK1, shPink1, and cells with combined shPink1 and shTBK1 (n = 3). The indicated A549 cells were initially induced with DMSO or doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. E) Immunoblot analysis of p53, p21, and Pink1 in the cells described in D (n = 3). F) Confocal imaging of Tom20 (green) and LAMP1 (red) in NC, shTBK1, shPink1, and cells with combined shPink1 and shTBK1 (n = 3). Cells were initially induced with doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. Areas outlined by squares in the merged images are enlarged at right. Scale bars, 2 μm.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence-Associated Pathologies.

doi: 10.1002/advs.202404109

Figure Lengend Snippet: Figure 7. TBK1-ATAD3A-Pink1 axis inhibits mitophagy and promotes cellular senescence. A) SA-𝛽-gal staining of indicated A549 cells (n = 3). Cells were initially induced with DMSO or doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. B) Immunoblot analysis of p53, p21, and Pink1 in the cells described in A (n = 3). C) Confocal imaging of Tom20 (green) and LAMP1 (red) in indicated cells (n = 3). Cells were initially induced with doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. Areas outlined by squares in the merged images are enlarged at right. Scale bars, 2 μm. D) SA-𝛽-gal staining of NC, shTBK1, shPink1, and cells with combined shPink1 and shTBK1 (n = 3). The indicated A549 cells were initially induced with DMSO or doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. E) Immunoblot analysis of p53, p21, and Pink1 in the cells described in D (n = 3). F) Confocal imaging of Tom20 (green) and LAMP1 (red) in NC, shTBK1, shPink1, and cells with combined shPink1 and shTBK1 (n = 3). Cells were initially induced with doxorubicin (150 nm) for 48 h, followed by additional culturing for 48 h. Areas outlined by squares in the merged images are enlarged at right. Scale bars, 2 μm.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATAD3A Abnova Cat#H00055210; RRID:AB_10718149 p-Ser/Thr Abcam Cat# ab17464; RRID:AB_443891 p21 Abcam Cat# ab109199; RRID:AB_10861551 p16 Abcam Cat# ab108349; RRID:AB_10858268 HA Abcam Cat# ab9110; RRID:AB_307019 LAMP1 Abcam Cat# ab25630; RRID:AB_470708 Ki-67 Abcam Cat# ab15580; RRID:AB_443209 VeriBlot for IP Detection Reagent (HRP) Abcam Cat# ab131366; RRID:AB_2892718 p-Parkin Abcam Cat# ab ab315376 HSC-70 Abcam Cat# ab19136 , RRID:AB_444764 TBK1 CST Cat# 38 066; RRID:AB_2827657 PLK1 CST Cat# 4513; RRID:AB_2167409 PKCδ CST Cat# 9616; RRID:AB_10949973 Cleaved caspase3 CST Cat# 9664; RRID:AB_2070042 PKM2 CST Cat# 4053; RRID:AB_1904096 PDH CST Cat# 3205; RRID:AB_2162926 GAPDH CST Cat# 2118; RRID:AB_561053 Tom20 Santa Cruz Cat# sc-11415; RRID:AB_2207533 Tim23 Santa Cruz Cat# sc-514463; RRID:AB_2923126 Tom40 Santa Cruz Cat# sc-365467; RRID:AB_10847086 GFP Santa Cruz Cat# sc-9996; RRID:AB_627695 TBK1 Santa Cruz Cat# sc-398366 β-Actin Santa Cruz Cat# sc-8432; RRID:AB_626630 Ubiquitin Santa Cruz Cat# sc-8017; RRID:AB_628423 OPTN Santa Cruz Cat# sc-166576; RRID:AB_2156554 p53 Santa Cruz Cat# sc-126; RRID:AB_628082 Flag Sigma Cat# F3165; RRID:AB_259529 Mitofilin Novus Cat#NB100-74461 ANT1 Proteintech Cat# 30631-1-AP Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31460 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Cat# 31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11008; RRID:AB_143165 Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Cat# A-11001; RRID:AB_2534069 Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-21207; RRID:AB_141637 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat# A-11037; RRID:AB_2534095 Chemicals Doxorubicin Selleck Cat# E2516 Recombinant TBK1 Protein Abcam Cat# ab85276 Protease Inhibitor Cocktail Roche Cat# P8340 Phosphatase inhibitor cocktail Roche Cat# PHOSS-RO (Continued) Adv.

Techniques: Staining, Western Blot, Imaging